Sunday, 24 April 2016
Hey everyone! Hope you're all well! I feel like all of my weeks are just blurring by as it's a similar routine every week and before I know it, it's the weekend again! Two of my best friends came round to visit this weekend and we had a lovely little catch up! Then during labs this past week I did a transfection. I basically have a gene of interest which I inserted into circular DNA (plasmid) and I put it into bacterial cells so they'd grow up loads up my DNA for me, I then extracted this DNA and mutated a few different base pairs. On Thursday I tried to insert this "bacterial" DNA into human cells and hopefully they took it up into their cells and expressed my gene of interest. So on Monday I am going to find out whether it actually worked or not and if there are any interesting results, so fingers crossed!
Sunday, 17 April 2016
Hey everyone! Hope you're all well! Labs are pretty much back in full swing so the long grueling days of DNA extractions have started again. I have to extract DNA from 350 samples and I'm currently on 230 I'm nearly there :') It's a bit of a pain as I can only really do 12 samples in a day and it takes forever!I should be done in the next few weeks though and then it's on to the next step :)